Start studying 13) Serial dilution agar plate. Spread plate method in which a sample containing. What is the advantages and disadvantages to the serial. Advantages And Disadvantages Of The Serial Dilution Agar Plate Technique. 8/3/2017 0 Comments MICROBIAL TESTING PROCEDURESMICROBIAL. TESTING PROCEDURES1. THE ADVANTAGES AND DISADVANTAGES OF. SPREAD PLATE TECHNIQUE A mixture of. It is time consuming especially when diluting the.

Contents • • • • Process [ ] The antibiotic to be tested is diluted with water to produce a series of concentrations. An appropriate volume is then combined with melted agar to produce plates in which the final antibiotic concentrations represent a 2-fold dilution series. After this, bacteria prepared to a standard concentration are added as a spot to each plate, with 10^4 colony forming units (CFU) per spot. This technique allows for replicate spots of one bacterial type to be tested or spots of different bacteria so that the MIC of the antibiotic against multiple types of bacteria can be tested.

Necessary controls include a control plate that does not receive any antibiotics and bacterial spread plates demonstrating that the bacterial inocula are in the correct concentration range. The dilution plates are then incubated at a temperature of 37 degrees. The plates are then incubated for sixteen to eighteen hours, although incubation time may be less for bacteria populations that divide quickly.: 374 After incubation, the plates are examined to determine if bacterial growth has occurred in the inoculated spots. The lowest concentration of antibiotics that prevented bacterial growth is considered to be the minimum inhibitory concentration of that antibiotic against that bacterium. Advantages [ ] Agar dilution is considered to be the of susceptibility testing, or the most accurate way to measure the resistance of bacteria to antibiotics.

The results of agar dilution are easily reproduced and they can be monitored at a much cheaper cost than what is required of other dilution methods. Free Vinnies Rampage Games Programs. Additionally, up to thirty pathogen samples (plus two controls) can be tested at once, so agar dilution is useful for batch tests.: 149 Disadvantages [ ] Each dilution plate in agar testing has to be manually inoculated with the pathogen to be tested, so agar dilution testing is both labor-intensive and expensive.: 149 Unlike tests, agar dilution cannot be used to test more than one antibiotic at a time. References [ ]. • ^ Lorian, Victor (2005)..

Lippincott Williams & Wilkins. Retrieved 16 November 2014. • Wiegand, Irith; Hilpert, Kai; Hancock, Robert E. 'Agar and broth dilution methods to determine the minimal inhibitory concentration (MIC) of antimicrobial substances'. Nature Protocols. Check date values in: date= (); access-date= requires url= () • European Committee for Antimicrobial Susceptibility Testing of the European Society of Clinical Microbiology and Infectious Disease (September 2000).

'Determination of minimum inhibitory concentrations (MICs) of antibacterial agents by agar dilution'. Clinical Microbiology and Infection.

6 (9): 509–515 Barudan Punchant Pro Manual on this page. .. access-date= requires url= () • ^ Parija (2009).. Elsevier India. Retrieved 20 November 2014. • Lee, Mary (2013). Retrieved 16 November 2014.

• Tell us some more • Upload in Progress • Upload failed. Please upload a file larger than 100x100 pixels • We are experiencing some problems, please try again. • You can only upload files of type PNG, JPG, or JPEG. • You can only upload files of type 3GP, 3GPP, MP4, MOV, AVI, MPG, MPEG, or RM. • You can only upload photos smaller than 5 MB. • You can only upload videos smaller than 600MB. • You can only upload a photo (png, jpg, jpeg) or a video (3gp, 3gpp, mp4, mov, avi, mpg, mpeg, rm).

• You can only upload a photo or a video. • Video should be smaller than 600mb/5 minutes • Photo should be smaller than 5mb •. Prinsip Good Corporate Governance Pdf there.