MTT test: Formation of formazan crystals from MTT in mesenchymal stem cells Formazan dyes are artificial products of the reduction of tetrazolium salts by and reductases. They have a variety of colors from dark blue to deep red to orange, depending on the original tetrazolium salt used as the substrate for the reaction. Leading examples of tetrazolium salts include: • or 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride, which is water-insoluble.

• or 3-(4,5-dimethyl-2-thiazolyl)-2, 5-diphenyl-2H-tetrazolium bromide, which is water-insoluble and used in the. • XTT or 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide, which is water-soluble. • or 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, which is water-soluble and used in the.

Title:Fluorescent Formazans and Tetrazolium Salts - Towards Fluorescent Cytotoxicity Assays. VOLUME: 19 ISSUE: 5. Author(s):Melissa K. Ladyman, Jeffrey G.A. 1.The red and purple formazans of NT. Download preview PDF. Studies on the reduction of tetrazolium salts.

• or 2,3,5-triphenyl-2H-tetrazolium chloride, which is water-soluble. • used in a diagnostic test, particularly for chronic granulomatous disease and other diseases of phagocyte function.

When reduced in a cell, either or through direct reaction with or, the classical tetrazolium salt, MTT, turns blue to purple and may form an insoluble precipitate. These formazan dyes are commonly used in cell proliferation and toxicity like the and tests since they only stain living, metabolically active cells.

Change Request Management Software Ware there. References [ ].